Ecological Politics and Practices in Introduced Species Management

The surveillance and control of introduced species has become an increasingly important, yet often controversial, form of environmental management. I investigate why and how introduced species management is initiated; whether, why and how it is contested; and what relations and outcomes emerge ‘in practice’. I examine how introduced species management is being done in the United Kingdom through detailed social scientific analyses of the processes, practices, and disputes involved in a series of management case studies. First, I demonstrate how some established approaches to the design and delivery of management initiatives can render them conflict-prone, ineffective and potentially unjust. Then, examining a disputesurrounding a state-initiated eradication of monk parakeets (Myiopsitta monachus), I show why and how ‘parakeet protectors’ opposed the initiative. I identify the significance of divergent evaluations of the risks posed by introduced wildlife; personal and community attachments between people and parakeets; and campaigners’ dissatisfaction with central government’s approach to the issue. By following the story of an unauthorised (re)introduction of Eurasian beavers (Castor fiber) to England, I show how adiverse collective has, at least temporarily, been united and empowered by a shared understanding of beavers as ‘belonging’ in the UK. I consider how nonhuman citizenship is socio-politically negotiated, and how the beavers have become enrolled in a ‘wild experiment’. Finally, through a multi- sited study of grey squirrel (Sciuruscarolinensis) control initiatives, I find important variations in management practitioners’ approaches to killing squirrels, and identify several ‘modes of killing’ that comprise different primary motivations, moral principles, ultimate aims, and practical methods. I identify multiple ways in which people respond and relate to introduced wildlife, and demonstrate how this multiplicity produces both socio-political tensions and accords. Furthermore, throughout this thesis I make a series of propositions for re-configuring the management of introduced species in ways that explicitly incorporate inclusive, constructive, and context-appropriate socio-political deliberations into its design and implementation.University of Exete

Examining the Effects of Parent Engagement on Early Childhood Development in Migrant-Seasonal Head Start

Several factors impact developmental outcomes in early childhood with implications that may last throughout the lifespan. Studies indicate that across most populations, parental engagement has a significant impact on relative success in early childhood development, such as higher literacy and language scores and socio-emotional skills. Identifying specific positive influences on early childhood development for caretakers to integrate in both the home and in the classroom is essential to creating policies and standards that support the healthy development of children. Knowledge concerning minority groups in this area remains particularly limited. This study investigated the influence of parental engagement on specific areas of development in children enrolled in a migrant-seasonal Head Start program. The hypothesis investigated conjectured a stronger positive correlation in Seasonal families than in migrant with a higher frequency of parent engagement being associated with higher benchmark scores. A binary logistic regression model was used to compare the level of documented parent engagement with the two family types’ composite engagement and benchmark achievement across five areas measured in Head Start: cognitive, social-emotional, physical, language and literacy, and mathematics. Findings are expected to enhance the migrant-seasonal program’s understanding of the parent-child relationship in two uniquely vulnerable minority groups, and to indicate that parent engagement is a critical component in healthy early childhood development, prompting further questions about the differences in lifestyle that may influence the relationship studied in this project

First Profunda Artery Perforator Flap for Breast Reconstruction Performed at a VA Hospital

The profunda artery perforator (PAP) flap for breast reconstruction originated by Dr. Robert J. Allen in 2010 after multiple failed attempts at using muscle-sparing transverse rectus abdominal muscle (MS-TRAM) flap and implants. This flap method was initially approached due to the patient’s request for an autologous tissue graft that did not sacrifice muscle. The PAP flap has grown significantly in popularity and usage in breast reconstruction and makes up to about 16% of autologous breast reconstruction, coming second to DIEP flaps (76%). This well-tolerated, muscle-sparing approach is ideal for patients without sufficient abdominal fat (low BMI or previous abdominal liposuction) or those who prefer a non-abdominal donor site. In addition to its gained popularity for breast reconstruction, the PAP flap has also been utilized in defects reconstruction over the entire body such as the head and neck, lower extremities, vulva, and tongue. This is a sophisticated procedure and has never been performed at a VA hospital before this case

The Deerfield Street Initiative (Greenfield, MA)

The goal of the Master of Regional Planning Studio is to develop a student’s techniques for collecting, analyzing, and synthesizing spatial and non-spatial data and then presenting that collective data in a manner (i.e., report, video, presentation, and charettes) that is understandable to academics, professionals, and the public. Planning Studio allows students to integrate knowledge from coursework and research, and apply such knowledge to resolving representative planning problems. At UMASS Amherst, these problems are found in neighborhood, rural, urban, and/or regional settings. For the fall 2018 Planning Studio, the Town of Greenfieldtasked the Masters of Regional Planning Studio to prepare a vision plan that focuses on improving Greenfield’sRoute 5 Southern/Deerfield Street Corridor. Greenfield’s Deerfield Street neighborhood serves as the southern gateway to the Downtown. This area has been in transition for several years as the City has invested in housing and infrastructure along this stretch. The key projects have been upgrade of sidewalks, creation of a small riverside park, renovation of distressed housing. Recently, the neighborhood has seen investment in new housing. The Arbors (constructed in 2007) is an upscale assisted housing residence thatalso has low-income housing units. The Green River Commons (2018) consists of eight newhigh performance (energy) modest-sized condominiums with units as fourlow-income housing. In addition, there are several multifamily homes have been or are scheduledfor rehabilitation under the City\u27s Housing Rehab Program

Regional natural resource management plan

[Extract] This plan builds on, updates and refines earlier NRM planning work in the region. The 2005 Southern Gulf Catchment Natural Resource Management Plan provided a comprehensive assessment of the natural resource values and issues in the Southern Gulf region and provided a long list of actions to address them. SG NRM’s work was aligned to this plan, but because the plan’s scope was so large, and because it did not provide a good guide for prioritising actions in the face of limited resources, the plan fell into abeyance. In 2014, the Australian Government provided funding to update the region’s NRM plan to incorporate climate change considerations through its Regional Natural Resource Management Planning for Climate Change Fund. Although there has been much progress in the region, many of NRM issues remain the same as they were in 2005. SG NRM therefore determined that the revised plan would draw on the good foundations of the earlier plan, but that its scope would be more strategic and its objectives more achievable. Therefore, it was decided that the scope of this plan is to• Update the 2005 Southern Gulf Catchment Natural Resource Management Plan • Incorporate climate change considerations• Acknowledge the range of NRM issues across in the Southern Gulf region, including issues that have arisen since 2005• Provide action plans to address these issues, focusing on the role of SG NRM and what it can achieve through its partnerships with the Southern Gulf community. SG NRM will use this plan as a guiding document to plan its investments and effort. The plan may also be used by Southern Gulf stakeholders to identify constructive approaches to addressing NRM issues in the region and potential partnerships with SG NRM. However, the plan does not commit either SG NRM or its partners to any specific action

A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose of this multi-laboratory study was to establish the precision and accuracy of basic data dimensions in AUC and validate previously proposed calibration techniques. Three kits of AUC cell assemblies containing radial and temperature calibration tools and a bovine serum albumin (BSA) reference sample were shared among 67 laboratories, generating 129 comprehensive data sets. These allowed for an assessment of many parameters of instrument performance, including accuracy of the reported scan time after the start of centrifugation, the accuracy of the temperature calibration, and the accuracy of the radial magnification. The range of sedimentation coefficients obtained for BSA monomer in different instruments and using different optical systems was from 3.655 S to 4.949 S, with a mean and standard deviation of (4.304 +/- 0.188) S (4.4%). After the combined application of correction factors derived from the external calibration references for elapsed time, scan velocity, temperature, and radial magnification, the range of s-values was reduced 7-fold with a mean of 4.325 S and a 6-fold reduced standard deviation of +/- 0.030 S (0.7%). In addition, the large data set provided an opportunity to determine the instrument-to-instrument variation of the absolute radial positions reported in the scan files, the precision of photometric or refractometric signal magnitudes, and the precision of the calculated apparent molar mass of BSA monomer and the fraction of BSA dimers. These results highlight the necessity and effectiveness of independent calibration of basic AUC data dimensions for reliable quantitative studies

Comparative analysis of microRNA expression in mouse and human brown adipose tissue

BACKGROUND: In small mammals brown adipose tissue (BAT) plays a predominant role in regulating energy expenditure (EE) via adaptive thermogenesis. New-born babies require BAT to control their body temperature, however its relevance in adults has been questioned. Active BAT has recently been observed in adult humans, albeit in much lower relative quantities than small mammals. Comparing and contrasting the molecular mechanisms controlling BAT growth and development in mice and humans will increase our understanding or how human BAT is developed and may identify potential therapeutic targets to increase EE. MicroRNAs are molecular mechanisms involved in mouse BAT development however, little is known about the miRNA profile in human BAT. The aims of this study were to establish a mouse BAT-enriched miRNA profile and compare this with miRNAs measured in human BAT. To achieve this we firstly established a mouse BAT enriched-miRNA profile by comparing miRNAs expressed in mouse BAT, white adipose tissue and skeletal muscle. Following this the BAT-enriched miRNAs predicted to target genes potentially involved in growth and development were identified. METHODS: MiRNA levels were measured using PCR-based miRNA arrays. Results were analysed using ExpressionSuite software with the global mean expression value of all expressed miRNAs in a givensample used as the normalisation factor. Bio-informatic analyses was used to predict gene targets followed by Ingenuity Pathway Analysis. RESULTS: We identified 35 mouse BAT-enriched miRNAs that were predicted to target genes potentially involved in growth and development. We also identified 145 miRNAs expressed in both mouse and human BAT, of which 25 were enriched in mouse BAT. Of these 25 miRNAs, miR-20a was predicted to target MYF5 and PPARγ, two important genes involved in brown adipogenesis, as well as BMP2 and BMPR2, genes involved in white adipogenesis. For the first time, 69 miRNAs were identified in human BAT but absent in mouse BAT, and 181 miRNAs were expressed in mouse but not in human BAT. CONCLUSION: The present study has identified a small sub-set of miRNAs common to both mouse and human BAT. From this sub-set bioinformatics analysis suggested a potential role of miR-20a in the control of cell fate and this warrants further investigation. The large number of miRNAs found only in mouse BAT or only in human BAT highlights the differing molecular profile between species that is likely to influence the functional role of BAT across species. Nevertheless the BAT-enriched miRNA profiles established in the present study suggest targets to investigate in the control BAT development and EE

Development of a standardized histopathology scoring system for intervertebral disc degeneration in rat models: An initiative of the ORS spine section

Background Rats are a widely accepted preclinical model for evaluating intervertebral disc (IVD) degeneration and regeneration. IVD morphology is commonly assessed using histology, which forms the foundation for quantifying the state of IVD degeneration. IVD degeneration severity is evaluated using different grading systems that focus on distinct degenerative features. A standard grading system would facilitate more accurate comparison across laboratories and more robust comparisons of different models and interventions. Aims This study aimed to develop a histology grading system to quantify IVD degeneration for different rat models. Materials & Methods This study involved a literature review, a survey of experts in the field, and a validation study using 25 slides that were scored by 15 graders from different international institutes to determine inter- and intra-rater reliability. Results A new IVD degeneration grading system was established and it consists of eight significant degenerative features, including nucleus pulposus (NP) shape, NP area, NP cell number, NP cell morphology, annulus fibrosus (AF) lamellar organization, AF tears/fissures/disruptions, NP-AF border appearance, as well as endplate disruptions/microfractures and osteophyte/ossification. The validation study indicated this system was easily adopted, and able to discern different severities of degenerative changes from different rat IVD degeneration models with high reproducibility for both experienced and inexperienced graders. In addition, a widely-accepted protocol for histological preparation of rat IVD samples based on the survey findings include paraffin embedding, sagittal orientation, section thickness < 10 μm, and staining using H&E and/or SO/FG to facilitate comparison across laboratories. Conclusion The proposed histological preparation protocol and grading system provide a platform for more precise comparisons and more robust evaluation of rat IVD degeneration models and interventions across laboratories